Mapping Dynamical Mechanical Properties of Osteonal Bone by Scanning Acoustic Microscopy in Time-of-Flight Mode

Autor(en)

Stephane Blouin, Stephan Puchegger, Andreas Roschger, Andrea Berzlanovich, Peter Fratzl, Klaus Klaushofer, Paul Roschger

Abstrakt

An important determinant of mechanical properties of bone is Young’s modulus and its variation in individual osteons of cortical bone tissue. Its mechanical behavior also depends on deformation rate owing to its visco- or poroelastic properties. We developed a method to measure dynamical mechanical properties of bulk bone tissue at osteonal level based on scanning acoustic microscopy (SAM) using time-of-flight (TOF) measurements in combination with quantitative backscattered electron imaging (qBEI). SAM-TOF yields local sound velocities and qBEI corresponding material densities together providing elastic properties. Osteons (n=55) were measured in three human femoral diaphyseal ground bone sections (∼30 µm in thickness). In addition, subchondral bone and mineralized articular cartilage were investigated. The mean mineral contents, the mean sound velocities, and the mean elastic modulus of the osteons ranged from 20 to 26 wt%, from 3,819 to 5,260 m/s, and from 21 to 44 GPa, respectively. There was a strong positive correlation between material density and sound velocity (Pearson’s r=0.701; p<0.0001) of the osteons. Sound velocities between cartilage and bone was similar, though material density was higher in cartilage (+4.46%, p<0.0001). These results demonstrate the power of SAM-TOF to estimate dynamic mechanical properties of the bone materials at the osteonal level.

Organisation(en)

Fakultätszentrum für Nanostrukturforschung

Externe Organisation(en)

Hanusch Krankenhaus, Medizinische Universität Wien, Max-Planck-Institut für Kolloid- und Grenzflächenforschung

Journal

Microscopy and Microanalysis

Band

20

Seiten

924-936

Anzahl der Seiten

13

ISSN

1431-9276

DOI

https://doi.org/10.1017/S1431927614000646

Publikationsdatum

06-2014

Peer-reviewed

Ja

ÖFOS 2012

103018 Materialphysik

Schlagwörter

ASJC Scopus Sachgebiete

Instrumentation

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/15ee899a-7e3c-4cf8-9fb1-3f67c4020eb6