Publikationen

Characterizing the Cell Surface Structure and Antibody Recognition Forces on Intact Microbial Cells using Scanning Probe Microscopy

Autor(en)
Yoo Jin Oh, Gerhard Sekot, Memed Duman, Lilia Chtcheglova, Paul Messner, Herwig Peterlik, Christina Schaeffer, Peter Hinterdorfer
Abstrakt

Tannerella forsythia is among the most potent triggers of periodontal diseases and approaches to understand underlying mechanisms are currently intensively pursued. A ∼22‑nm thick, 2D crystallinesurface (S‑) layer that completely covers T. forsythia cells is crucially involved in the bacterium-host cross-talk. The S-layer is composed of two intercalating glycoproteins (TfsA‑GP, TfsB‑GP) that are aligned into a periodic lattice. To characterize this unique S‑layer structure at the nanometer-scale directly on intact T. forsythia cells, atomic force microscopy (AFM) based topography imaging and single-molecular force spectroscopy (SMFS) were applied.

Organisation(en)
Dynamik Kondensierter Systeme, Fakultätszentrum für Nanostrukturforschung
Externe Organisation(en)
Johannes Kepler Universität Linz, Universität für Bodenkultur Wien, Ctr Adv Bioanal CBL
Journal
Biophysical Journal
Band
106
Seiten
511A-512A
Anzahl der Seiten
2
ISSN
0006-3495
DOI
https://doi.org/10.1016/j.bpj.2013.11.2859
Publikationsdatum
01-2014
Peer-reviewed
Ja
ÖFOS 2012
Biophysik
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/characterizing-the-cell-surface-structure-and-antibody-recognition-forces-on-intact-microbial-cells-using-scanning-probe-microscopy(a4db5931-caa2-4946-b8b9-832712231e41).html